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An 8-wk experiment was conducted to determine the optimal protein requirement of juvenile black sea bream, Sparus macrocephalus. (2004) in freshwater crayfish (Cherax quadricarinatus) found low PER.

(2004) with slight modification. Briefly, infected gills were homogenized in PBS (2.7 mm KCl, 137 mm NaCl, 10 mm Na 2 HPO 4 , 2 mm KH 2 PO 4 , pH 7.4) at 4 °C with a glass tissue blender.

1998), hemocytes of the crayfish Procambarus clarkii following exposure to bacterial LPS (Yeh et al., 2006), hemocytes of the mussel Mytilus galloprovincinalis after immune challenge by PAMPs and live.

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Special Publication No. 9, Bredene, Belgium. van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Vago C. (1966) A virus disease in Crustacea. Nature 209, 1290. Wongteerasupaya C., Vickers J.E.,

(2004) with slight modification. Briefly, infected gills were homogenized in PBS (2.7 mm KCl, 137 mm NaCl, 10 mm Na 2 HPO 4 , 2 mm KH 2 PO 4 , pH 7.4) at 4 °C with a glass tissue blender.

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An 8-wk experiment was conducted to determine the optimal protein requirement of juvenile black sea bream, Sparus macrocephalus. (2004) in freshwater crayfish (Cherax quadricarinatus) found low PER.

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